Investigations of Some Reliable Electrochemiluminescence Systems on the Basis of tris(bipyridyl)Ruthenium(II) for HPLC Analysis

A. Habekost^1,

¹Department of Chemistry, University of Education Ludwigsburg, Reuteallee 46, D-71634 Ludwigsburg, Germany

Cite this paper

A. Habekost. Investigations of Some Reliable Electrochemiluminescence Systems on the Basis of tris(bipyridyl)Ruthenium(II) for HPLC Analysis. World Journal of Chemical Education. 2016; 4(1):13-20. doi: 10.12691/WJCE-4-1-3

Abstract

Electrogenerated chemiluminescence (ECL) is not only an aesthetic phenomenon, but also of fundamental interest in analytical chemistry Several experiments are presented to introduce students to the capacity of ECL on the basis of tris(2, 2’-bipyridyl)ruthenium (II) (Ru(bpy)₃²⁺) with different coreactants.. First, we summarize some spectroscopic and electrochemical features of Ru(bpy)₃²⁺ that are important concerning ECL. Easily implemented experimental set-ups are presented, which show the correlation between ECL-intensity and cyclic voltammetry (CV) for different Ru(bpy)₃²⁺ / coreactant systems. The time-dependence of the ECL-decay is measured on a millisecond time-scale with a conventional data acquisition system. Finally, we present an ECL detector that can be used in HPLC as an alternative to adsorption and fluorescence detection.

Keywords

four-year undergraduate, beginner PhD student, analytical, UVVIS and fluorescence spectroscopy, mass spectrometry, electrochemistry, chemiluminescence, HPLC, hands-on learning/manipulatives

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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