Bovine Serum Albumin Bioconjugation with FITC

Nadia Barbero^1, , Claudia Barolo¹ and Guido Viscardi¹

¹University of Torino, Department of Chemistry and NIS and INSTM Reference Centre, Via Pietro Giuria 7, 10125, Torino, Italy

Cite this paper

Nadia Barbero, Claudia Barolo and Guido Viscardi. Bovine Serum Albumin Bioconjugation with FITC. World Journal of Chemical Education. 2016; 4(4):80-85. doi: 10.12691/WJCE-4-4-3

Abstract

This laboratory provides students a basic approach to bioconjugation experiments, a topic which is not typically covered in didactical laboratories. Students are required to characterize a fluorescent probe as well as a protein and then to perform a bioconjugate experiment. The resulting marked protein is characterized by means of UV-Vis spectroscopy.

Keywords

bioconjugation, bovine serum Albumin, FITC, UV-Vis spectroscopy

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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