World Journal of Chemical Education. 2017, 5(3), 102-106
DOI: 10.12691/WJCE-5-3-4
The Inhibition and Reactivation of Enzymatic Processes
A. Habekost1,
1University of Education Ludwigsburg, Department of Chemistry, Reuteallee 46, D-71634 Ludwigsburg, Germany
Pub. Date: May 02, 2017
Cite this paper
A. Habekost. The Inhibition and Reactivation of Enzymatic Processes.
World Journal of Chemical Education. 2017; 5(3):102-106. doi: 10.12691/WJCE-5-3-4
Abstract
We present experiments that show the inhibition of the catalytic activity of the enzyme urease on the chemical degradation of urea with copper ions. In addition, we demonstrate the efficiency of the antidotes 2,3-dimercapto-1-propanesulfonic acid (DMPS, 2,3-bis(sulfanyl)propane-1-sulfonic acid) and 2,5-dimercapto-1,3,4-thiadiazole (DMTD) on reactivation of urease by complexation of copper.
Keywords
three-year undergraduate, analytical, electrochemistry, electrochemiluminescence, IR-spectrometry, hands-on learning/manipulatives
Copyright
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References
[1] | C.A McAuliffe, S.G Murray, Metal complexes of sulphur-containing amino acids, Inorg. Chim. Acta Rev. 6, 103-121 (1972). |
|
[2] | B. Vallant, J. Deutsch, M. Muntean, W. Goessler, Intravenous injection of metallic mercury: case report and course of mercury during chelation therapy with DMPS, Clinical toxicology (Philadelphia, Pa.), 46(6), 566-56, (2008). |
|
[3] | G. Drasch, S. Boese-O’Reilly, S. Illig, Increase of renal excretion of organo-mercury compounds like methylmercury by DMPS (2,3-Dimercapto-1-propanesulfonic acid, Dimaval), Clinical Toxicology, 45(3), 266-269 (2007). |
|
[4] | G. N. George, R. C. Prince, J. Gailer, G. A. Buttigieg, M. B. Denton, H. H. Harris, I. J. Pickering, Mercury binding to the chelation therapy agents DMSA and DMPS and the rational design of custom chelators for mercury, Chem. Res. in Tox., 17(8), 999-1006 (2004). |
|
[5] | H. V. Aposhian, R. M. Maiorino, D. Gonzalez-Ramirez, M. Zuniga-Charles, Z. Xu, K. M. Hurlbut, P. Junco-Munoz, R. C. Dart, M. M. Aposhian, Mobilization of heavy metals by newer, therapeutically useful chelating agents, Toxicology, 97(1-3), 23-38 (1995). |
|
[6] | A. Habekost, Investigations of some reliable electrochemiluminescence systems on the basis of tris(bipyridyl)Ruthenium(II) for HPLC analysis, World J. Chem. Educ. 4/1, 13-20 (2016). |
|